Ultra-LEAF™ Purified anti-mouse CD152 Antibody, CD152, 9H10,BioLegend,106213

Additional reported applications (for the relevant formats) include:in vivoandin vitroblocking1-4of CTLA-4 action by soluble antibody and promotion ofin vitroCTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No, 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos.106211 - 106216).

Host

Syrian Hamster

Reactivity

Mouse

Application

ELISA -Quality testedFC, Block, Costim -Reported in the literature, not verified in house

Platform ID

BAB388858989

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse CD152 Antibody, CD152, 9H10
Cat. No.106213
HostSyrian Hamster
RRIDAB_2813939 (BioLegend Cat. No. 106211)AB_2813940 (BioLegend Cat. No. 106212)AB_2813941 (BioLegend Cat. No. 106213)AB_2813942 (BioLegend Cat. No. 106214)AB_2813943 (BioLegend Cat. No. 106215)AB_2813944 (BioLegend Cat. No. 106216)
IsotypeSyrian Hamster IgG
ReactivityMouse
ApplicationELISA -Quality testedFC, Block, Costim -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number9H10
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD152
ImmunogenMouse CTLA-4-human IgG1 fusion protein
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.

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