Ultra-LEAF Purified anti-mouse CD178 FasL Antibody, CD178, MFL3,BioLegend,106612

Additional reported applications (for the relevant formats) include: inhibition of the cytotoxicity1-3and immunofluorescence microscopy4. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106604) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106612).

Host

Armenian Hamster

Reactivity

Mouse

Application

FC -Quality testedBlock, Apop, ICC -Reported in the literature, not verified in house

Platform ID

BAB815421727

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse CD178 FasL Antibody, CD178, MFL3
Cat. No.106612
HostArmenian Hamster
RRIDAB_2813953 (BioLegend Cat. No. 106611)AB_2813954 (BioLegend Cat. No. 106612)
IsotypeArmenian Hamster IgG
ReactivityMouse
ApplicationFC -Quality testedBlock, Apop, ICC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMFL3
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD178
ImmunogenB6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

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