Ultra-LEAF™ Purified anti-mouse CD18 Antibody, CD18, M18/2,BioLegend,101420
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,in vitroandin vivoblocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101417-101422).
Host
Rat
Reactivity
Mouse
Application
FC -Quality testedIP, WB, Block, Stim, IHC -Reported in the literature, not verified in house
Platform ID
BAB685661513
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse CD18 Antibody, CD18, M18/2
Cat. No.101420
HostRat
RRIDAB_2832273 (BioLegend Cat. No. 101417)AB_2832274 (BioLegend Cat. No. 101418)AB_2832275 (BioLegend Cat. No. 101419)AB_2832276 (BioLegend Cat. No. 101420)AB_2832277 (BioLegend Cat. No. 101421)AB_2832278 (BioLegend Cat. No. 101422)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedIP, WB, Block, Stim, IHC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberM18/2
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD18
ImmunogenMouse CTL glycoprotein
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
CD18 is a 95 kD protein, also known as integrin β2subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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