Ultra-LEAF Purified anti-mouse CD25 Antibody anti-CD25 - PC61,BioLegend,102059
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,in vitroblocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4,in vivodepletion of CD25+CD4+Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Host
Rat
Reactivity
Mouse
Application
FC -Quality testedIHC-F, IP, Block, Depletion -Reported in the literature, not verified in house
Platform ID
BAB766234084
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse CD25 Antibody anti-CD25 - PC61
Cat. No.102059
HostRat
RRIDAB_2813922 (BioLegend Cat. No. 102057)AB_11150394 (BioLegend Cat. No. 102040)AB_2813923 (BioLegend Cat. No. 102058)AB_2813924 (BioLegend Cat. No. 102059)AB_2813925 (BioLegend Cat. No. 102060)AB_2813926 (BioLegend Cat. No. 102061)
IsotypeRat IgG1, λ
ReactivityMouse
ApplicationFC -Quality testedIHC-F, IP, Block, Depletion -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberPC61
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD25
ImmunogenIL-2-dependent cytolytic mouse T-cell clone B6.1
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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