Ultra-LEAF™ Purified anti-mouse CD253 (TRAIL) Antibody, CD253, N2B2,BioLegend,109315
Additional reported applications (for the relevant formats) include:in vitroblocking of NK cell cytotoxicity1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109308).
Host
Rat
Reactivity
Mouse
Application
FC -Qualit testedBlock -Reported in the literature, not verified in house
Platform ID
BAB258587756
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse CD253 (TRAIL) Antibody, CD253, N2B2
Cat. No.109315
HostRat
RRIDAB_2813976 (BioLegend Cat. No. 109315)AB_2813977 (BioLegend Cat. No. 109316)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Qualit testedBlock -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberN2B2
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD253
ImmunogenMouse TRAIL-transfected 2PK-3 cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicityin vitro.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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