Ultra-LEAF Purified anti-mouse CD279 PD-1 Antibody anti-CD279 - RMP1-14,BioLegend,114109
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114108). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 114110) with a lower endotoxin limit than standard LEAF™ purified antibodies.
Host
Rat
Reactivity
Mouse
Application
FC - Quality testedIHC, WB, FA - Reported in the literature, not verified in house
Platform ID
BAB961274213
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse CD279 PD-1 Antibody anti-CD279 - RMP1-14
Cat. No.114109
HostRat
RRIDAB_2783054 (BioLegend Cat. No. 114109)AB_2564183 (BioLegend Cat. No. 114110)AB_2800575 (BioLegend Cat. No. 114119)AB_2800576 (BioLegend Cat. No. 114120)AB_2800577 (BioLegend Cat. No. 114121)AB_2800578 (BioLegend Cat. No. 114122)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality testedIHC, WB, FA - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberRMP1-14
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD279
ImmunogenMouse PD-1 transfected BHK cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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