Ultra-LEAF Purified anti-mouse CD28 Antibody, CD28, E18,BioLegend,122021
Additional reported applications (for the relevant formats) include:in vitrocostimulation of T and NK cells1,in vitroblocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, andin vitroinduction of thymocyte differentiation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 122004).
Host
Mouse
Reactivity
Mouse
Application
FC -Quality testedCostim, Block -Reported in the literature, not verified in house
Platform ID
BAB595993310
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse CD28 Antibody, CD28, E18
Cat. No.122021
HostMouse
RRIDAB_2810370 (BioLegend Cat. No. 122021)AB_2810371 (BioLegend Cat. No. 122022)
IsotypeMouse IgG2b, κ
ReactivityMouse
ApplicationFC -Quality testedCostim, Block -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberE18
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD28
ImmunogenA20 cells and recombinant mCD28Ig
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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