Ultra-LEAF Purified anti-mouse CD28 Antibody anti-CD28 - 37.51,BioLegend,102132
Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrocostimulation of T and NK cells1,in vitroblocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4,in vitroinduction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).
Host
Syrian Hamster
Reactivity
Mouse
Application
FC -Quality testedIP, IHC-F, Costim, Block -Reported in the literature, not verified in house
Platform ID
BAB743856095
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse CD28 Antibody anti-CD28 - 37.51
Cat. No.102132
HostSyrian Hamster
RRIDAB_11150408 (BioLegend Cat. No. 102115)AB_11147170 (BioLegend Cat. No. 102116)AB_2810330 (BioLegend Cat. No. 102121)AB_2810331 (BioLegend Cat. No. 102122)AB_2810332 (BioLegend Cat. No. 102131)AB_2810333 (BioLegend Cat. No. 102132)
IsotypeSyrian Hamster IgG
ReactivityMouse
ApplicationFC -Quality testedIP, IHC-F, Costim, Block -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number37.51
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD28
ImmunogenC57BL/6 mouse T-cell lymphoma EL-4
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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