Ultra-LEAF™ Purified anti-mouse CD31 Antibody, CD31, MEC13.3,BioLegend,102529
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).
Host
Rat
Reactivity
Mouse
Application
FC -Quality testedIP, Block, IHC-F -Reported in the literature, not verified in house
Platform ID
BAB002065888
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse CD31 Antibody, CD31, MEC13.3
Cat. No.102529
HostRat
RRIDAB_2832292 (BioLegend Cat. No. 102529)AB_2832293 (BioLegend Cat. No. 102530)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedIP, Block, IHC-F -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMEC13.3
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD31
ImmunogenPolyoma middle T transformed EC line tEnd.1
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
This product is eligible for a 40% discount!To take advantage of this offer,please contact your local representative. This promotion is valid through April 30th. Exclusions apply. To view full promotion terms and conditions, visit ourPromotions page.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
Request a product
Please provide the required information below so that we can quickly source your products.