Ultra-LEAF Purified anti-mouse CD4 Antibody anti-CD4 - GK1.5,BioLegend,100471
Additional reported applications (for the relevant formats) include: blocking of CD4+T cell activation1,4,11, thymocyte costimulation3,in vitroandin vivodepletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Application
FC - Quality testedBlock, Costim, Depletion, IHC, IP - Reported in the literature, not verified in house
Platform ID
BAB100141318
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse CD4 Antibody anti-CD4 - GK1.5
Cat. No.100471
HostRat
RRIDAB_11150580 (BioLegend Cat. No. 100441)AB_11149488 (BioLegend Cat. No. 100442)AB_2810318 (BioLegend Cat. No. 100457)AB_2810319 (BioLegend Cat. No. 100458)AB_2810320 (BioLegend Cat. No. 100470)AB_2810321 (BioLegend Cat. No. 100471)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality testedBlock, Costim, Depletion, IHC, IP - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberGK1.5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD4
ImmunogenMouse CTL clone V4
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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