Ultra-LEAF Purified anti-mouse CD45 Antibody, CD45, 30-F11,BioLegend,103163
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Host
Rat
Reactivity
Mouse
Application
FC -Quality testedCyTOF® -VerifiedIP, CMCD, IHC, WB -Reported in the literature, not verified in house
Platform ID
BAB992481755
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse CD45 Antibody, CD45, 30-F11
Cat. No.103163
HostRat
RRIDAB_2819789 (BioLegend Cat. No. 103163)AB_2819790 (BioLegend Cat. No. 103164)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC -Quality testedCyTOF® -VerifiedIP, CMCD, IHC, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number30-F11
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD45
ImmunogenMouse thymus or spleen
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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