Ultra-LEAF Purified anti-mouse CD47 Antibody anti-CD47 - miap301,BioLegend,127519

Additional reported applications (for the relevant formats) include: blocking2and immunohistochemical staining of frozen sections3. This clone is not cross-reactive with human CD47.

Host

Rat

Reactivity

Mouse

Application

FC - Quality testedBlock - Reported in the literature, not verified in house

Platform ID

BAB941583669

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse CD47 Antibody anti-CD47 - miap301
Cat. No.127519
HostRat
RRIDAB_2810387 (BioLegend Cat. No. 127520)AB_2571996 (BioLegend Cat. No. 127517)AB_2571997 (BioLegend Cat. No. 127518)AB_2571998 (BioLegend Cat. No. 127519)AB_2810388 (BioLegend Cat. No. 127533)AB_2810389 (BioLegend Cat. No. 127534)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality testedBlock - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Numbermiap301
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD47
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

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