Ultra-LEAF Purified anti-mouse CD49d Antibody, CD49d, 9C10 (MFR4.B),BioLegend,103710

Additional reported applications (for the relevant formats) include:in vitroblocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3of acetone-fixed frozen sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103709 & 103710).

Host

Rat

Reactivity

Mouse

Application

FC -Quality testedBlock, IHC-F -Reported in the literature, not verified in house

Platform ID

BAB699666052

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse CD49d Antibody, CD49d, 9C10 (MFR4.B)
Cat. No.103710
HostRat
RRIDAB_2832316 (BioLegend Cat. No. 103709)AB_2832317 (BioLegend Cat. No. 103710)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedBlock, IHC-F -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number9C10 (MFR4.B)
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD49d
Immunogen(C57BL/6 x A/J)F1mouse fetal liver mas
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD49d is a 150 kD glycoprotein, also known as α4integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The 9C10(MFR4.B) antibody, in combination with the R1-2 monoclonal antibody, completely blocks VCAM-1 binding to VLA-4.

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