Ultra-LEAF Purified anti-mouse CD54 Antibody, CD54, YN1174,BioLegend,116132

Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Host

Rat

Reactivity

Mouse

Application

FC -Quality testedIP, WB -Reported in the literature, not verified in house

Platform ID

BAB665859758

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse CD54 Antibody, CD54, YN1174
Cat. No.116132
HostRat
RRIDAB_2813980 (BioLegend Cat. No. 116131)AB_2813981 (BioLegend Cat. No. 116132)AB_2813982 (BioLegend Cat. No. 116133)AB_2813983 (BioLegend Cat. No. 116134)AB_2813984 (BioLegend Cat. No. 116135)AB_2813985 (BioLegend Cat. No. 116136)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC -Quality testedIP, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberYN1/1.7.4
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD54
ImmunogenMouse NS-1 cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

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