Ultra-LEAF Purified anti-mouse CD8a Antibody, CD8a, 53-67,BioLegend,100777
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Application
FC - Quality testedCyTOF® - VerifiedIHC, IP, Depletion, Block - Reported in the literature, not verified in house
Platform ID
BAB001777291
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse CD8a Antibody, CD8a, 53-67
Cat. No.100777
HostRat
RRIDAB_2810325 (BioLegend Cat. No. 100775)AB_11147171 (BioLegend Cat. No. 100746)AB_2810323 (BioLegend Cat. No. 100763)AB_2810324 (BioLegend Cat. No. 100764)AB_2810326 (BioLegend Cat. No. 100776)AB_2810327 (BioLegend Cat. No. 100777)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality testedCyTOF® - VerifiedIHC, IP, Depletion, Block - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number53-6.7
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD8alpha
ImmunogenMouse thymus or spleen
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
This product is eligible for a 40% discount!To take advantage of this offer,please contact your local representative. This promotion is valid through April 30th. Exclusions apply. To view full promotion terms and conditions, visit ourPromotions page.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
Request a product
Please provide the required information below so that we can quickly source your products.