Ultra-LEAF™ Purified anti-mouse GM-CSF Antibody, GM-CSF, MP1-22E9,BioLegend,505415
ELISA or ELISPOT Capture1,3-5:The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry8:The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.Neutralization2-4:The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended forin vivoandin vitroneutralization (Cat. No. 505408).Additional reported applications (for the relevant formats) include:immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,6,7, and immunocytochemistry8.
Host
Rat
Reactivity
Mouse
Application
ELISA Capture -Quality testedELISPOT Capture, Neut, ICC, IHC-F -Reported in the literature, not verified in house
Platform ID
BAB256288860
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse GM-CSF Antibody, GM-CSF, MP1-22E9
Cat. No.505415
HostRat
RRIDAB_2810635 (BioLegend Cat. No. 505415)AB_2810636 (BioLegend Cat. No. 505416)AB_2810637 (BioLegend Cat. No. 505417)AB_2810638 (BioLegend Cat. No. 505418)AB_2810639 (BioLegend Cat. No. 505419)AB_2810640 (BioLegend Cat. No. 505420)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationELISA Capture -Quality testedELISPOT Capture, Neut, ICC, IHC-F -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMP1-22E9
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetGM-CSF
ImmunogenYeast-expressed, recombinant mouse GM-CSF
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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