Ultra-LEAF™ Purified anti-mouse I-A/I-E Antibody, I-A/I-E, M5/114.15.2,BioLegend,107655
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Host
Rat
Reactivity
Mouse
Application
FC -Quality testedIHC-F, IP, Block -Reported in the literature, not verified in house
Platform ID
BAB499117304
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse I-A/I-E Antibody, I-A/I-E, M5/114.15.2
Cat. No.107655
HostRat
RRIDAB_2813968 (BioLegend Cat. No. 107655)AB_2813969 (BioLegend Cat. No. 107656)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC -Quality testedIHC-F, IP, Block -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberM5/114.15.2
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetI-A/I-E
ImmunogenActivated C57BL/6 mouse spleen cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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