Ultra-LEAF™ Purified anti-mouse IFN-γ Antibody, IFN-γ, AN-18,BioLegend,517906
ELISA Capture:The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detecting antibody, and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: immunoprecipitation.
Host
Rat
Reactivity
Mouse
Application
ELISA Capture -Quality testedELISPOT Capture, IP -Reported in the literature, not verified in house
Platform ID
BAB142832081
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse IFN-γ Antibody, IFN-γ, AN-18
Cat. No.517906
HostRat
RRIDAB_2810651 (BioLegend Cat. No. 517905)AB_2810652 (BioLegend Cat. No. 517906)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationELISA Capture -Quality testedELISPOT Capture, IP -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberAN-18
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIFN-gamma
ImmunogenMouse IFN-γ protein
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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