Ultra-LEAF™ Purified anti-mouse IL-1α Antibody, IL-1α, ALF-161,BioLegend,503212

ELISA or ELISPOT Capture:The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5034 antibody (Cat. No. 503401) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.ELISA Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated BL1a-89 antibody (Cat. Nos. 512503 & 512504) as the detection antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.Neutralization:Clone ALF-161 has been described to have IL-1a neutralizing properties based on thein vivomice model3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended (Cat. No. 503207-503212).

Host

Armenian Hamster

Reactivity

Mouse

Application

ELISA Capture -Quality testedELISPOT Capture, Neut, ICFC, WB, IP -Reported in the literature, not verified in house

Platform ID

BAB455238376

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse IL-1α Antibody, IL-1α, ALF-161
Cat. No.503212
HostArmenian Hamster
RRIDAB_2861033 (BioLegend Cat. No. 503207)AB_2861034 (BioLegend Cat. No. 503208)AB_2861035 (BioLegend Cat. No. 503209)AB_2861036 (BioLegend Cat. No. 503210)AB_2861037 (BioLegend Cat. No. 503211)AB_2876737 (BioLegend Cat. No. 503212)
IsotypeArmenian Hamster IgG
ReactivityMouse
ApplicationELISA Capture -Quality testedELISPOT Capture, Neut, ICFC, WB, IP -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberALF-161
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-1alpha
ImmunogenE. coli- expressed, recombinant mouse IL-1
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The ALF-161 antibody reacts with precursor, secreted and membrane-associated forms of mouse IL-1α. The ALF-161 antibody can neutralize the bioactivity of natural or recombinant IL-1α.

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