Ultra-LEAF Purified anti-mouse IL-12/IL-23 p40 monomer, dimer, heterodimer Antibody anti-IL-12/IL-23 p40 - C17.8,BioLegend,505307
ELISA Capture or ELISpot Capture: The C17.8 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay.Neutralization1,2,4,5,6,10:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-12 bioactivityin vivoandin vitro(Cat. No. 505304). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for relevant formats of this clone) include:Western blotting3, immunoprecipitation3, and intracellular immunofluorescent staining and flow cytometric analysis.Note:For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Application
ELISA Capture - Quality testedNeut, ICFC, IP, WB - Reported in the literature, not verified in house
Platform ID
BAB275690807
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse IL-12/IL-23 p40 monomer, dimer, heterodimer Antibody anti-IL-12/IL-23 p40 - C17.8
Cat. No.505307
HostRat
RRIDAB_11150770 (BioLegend Cat. No. 505307)AB_11148948 (BioLegend Cat. No. 505308)AB_2783330 (BioLegend Cat. No. 505309)AB_2783331 (BioLegend Cat. No. 505310)AB_2783332 (BioLegend Cat. No. 505311)AB_2783333 (BioLegend Cat. No. 505312)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationELISA Capture - Quality testedNeut, ICFC, IP, WB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberC17.8
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-12/IL-23 p40
ImmunogenCHO expressed, recombinant mouse IL-12 p70
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
The C17.8 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C17.8 antibody can neutralize the bioactivity of natural or recombinant IL-12.
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