Ultra-LEAF™ Purified anti-mouse IL-25 (IL-17E) Antibody, IL-25, 35B,BioLegend,514417

Neutralization: The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-25 bioactivity (Cat. No. 514417).ELISA Capture: The 35B antibody is useful as a capture antibody for a mouse IL-25 (IL-17E) sandwich ELISA assay, when used in conjunction with biotinylated M3704D11 antibody (Cat. No. 538104) as the detection antibody.

Host

Rat

Reactivity

Mouse

Application

ELISA Capture - Quality testedNeut - Reported in the literature, not verified in house

Platform ID

BAB243606215

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse IL-25 (IL-17E) Antibody, IL-25, 35B
Cat. No.514417
HostRat
RRIDAB_2814443 (BioLegend Cat. No. 514417)AB_2814444 (BioLegend Cat. No. 514418)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationELISA Capture - Quality testedNeut - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number35B
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-25
ImmunogenIL-25
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

IL-25 (IL-17E) is a member of the IL-17 family of proinflammatory cytokines which include at least six members including IL-17A (IL-17), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F. IL-17E is found at low levels in various peripheral tissues. It is a proinflammatory cytokine that promotes airway eosinophilia in mice. IL-17E plays a role in Th2 type inflammatory diseases, such as asthma and atopic dermatitis.

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