Ultra-LEAF Purified anti-mouse IL-27 p28 Antibody anti-IL-27 - MM27-7B1,BioLegend,516912

Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.1We confirmed clone MM27-7B1 cross-reactivity to recombinant human IL-27 in-house using an immunoassay.ELISA Capture:To measure mouse IL-27, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated B02P6E6 antibody (Cat. No. 525904) as the detection antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.Flow Cytometry:The fluorochrome-labeled MM27-7B1 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.Note:For measuringmouse IL-27 in serum, plasma or cell culture supernatant,LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kitwith Pre-coated Plates (Cat. No.438707&438708) are specially developed and recommended.

Host

Mouse

Reactivity

Mouse, Human

Application

ELISA Capture -Quality testedNeut -Reported in the literature, not verified in house

Platform ID

BAB005964016

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse IL-27 p28 Antibody anti-IL-27 - MM27-7B1
Cat. No.516912
HostMouse
RRIDAB_2561634 (BioLegend Cat. No. 516912)
IsotypeMouse IgG2a
ReactivityMouse, Human
ApplicationELISA Capture -Quality testedNeut -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMM27-7B1
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-27
ImmunogenMouse IL-27-OVA
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

IL-27 is a heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 P35-related protein). It is a member of the IL-6/IL-12 cytokine family and mainly produced by antigen-presenting cells, including macrophages and dendritic cells. IL-27 acts on T cells and NK cells. It has been reported that IL-27 drives rapid clonal expansion of naïve CD4+T cells, and promotes Th1 polarization and IFN-γ production in synergy with IL-12. The IL-27-induced Th1 differentiation was mediated by rapid and marked upregulation of ICAM-1/LFA-1 interaction in a STAT1-dependent manner. IL-27 exhibits anti-inflammatory function by enhancing Th1 cell differentiation, a potent antitumor activity, through CD8+T cell and NK cell activation. It also plays a potential therapeutic role in autoimmune disease by inhibiting Th-17 development. IL-27 mediates its biological effects through its receptor, WSX-1/T cell cytokine receptor (TCCR), which is homologous to the IL-12Rβ2 subunit. Protein gp130 serves as a functional signal-transducing molecule for IL-27.

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