Ultra-LEAF™ Purified anti-mouse IL-3 Antibody, IL-3, MP2-8F8,BioLegend,503912
ELISA or ELISPOT Capture1-5,8:The purified MP2-8F8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP2-43D11 antibody (Cat. No. 504002) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Host
Rat
Reactivity
Mouse
Application
ELISA Capture -Quality testedELISPOT Capture, Neut -Reported in the literature, not verified in house
Platform ID
BAB506413800
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse IL-3 Antibody, IL-3, MP2-8F8
Cat. No.503912
HostRat
RRIDAB_2890852 (BioLegend Cat. No. 503909)AB_2890852 (BioLegend Cat. No. 503910)AB_2890852 (BioLegend Cat. No. 503911)AB_2890852 (BioLegend Cat. No. 503912)AB_2890852 (BioLegend Cat. No. 503913)AB_2890852 (BioLegend Cat. No. 503914)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationELISA Capture -Quality testedELISPOT Capture, Neut -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMP2-8F8
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-3
ImmunogenCOS-expressed, recombinant mouse IL-3
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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