Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, Ly-6G/Ly-6C, RB6-8C5,BioLegend,108454
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytesin vivo. Additional reported applications (for relevant formats of this clone) include:in vitrocomplement-mediated cytotoxicity2,in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6(including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.Special Note:Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).
Host
Rat
Reactivity
Mouse
Application
FC - Quality testedIP, CMCD, Depletion, IHC, WB - Reported in the literature, not verified in house
Platform ID
BAB646868878
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, Ly-6G/Ly-6C, RB6-8C5
Cat. No.108454
HostRat
RRIDAB_11148945 (BioLegend Cat. No. 108435)AB_11147952 (BioLegend Cat. No. 108436)AB_2616681 (BioLegend Cat. No. 108453)AB_2616682 (BioLegend Cat. No. 108454)AB_2876420 (BioLegend Cat. No. 108461)AB_2876420 (BioLegend Cat. No. 108462)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality testedIP, CMCD, Depletion, IHC, WB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberRB6-8C5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetLy-6G Ly-6C
ImmunogenRaised against granulocytes of mouse origin
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
This product is eligible for a 40% discount!To take advantage of this offer,please contact your local representative. This promotion is valid through April 30th. Exclusions apply. To view full promotion terms and conditions, visit ourPromotions page.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
Request a product
Please provide the required information below so that we can quickly source your products.