Ultra-LEAF Purified anti-mouse Ly49H Antibody, Ly49H, 3D10,BioLegend,144717

Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, andin vitro2andin vivo3blocking activity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).

Host

Mouse

Reactivity

Mouse

Application

FC -Quality testedIP, Block -Reported in the literature, not verified in house

Platform ID

BAB307686706

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse Ly49H Antibody, Ly49H, 3D10
Cat. No.144717
HostMouse
RRIDAB_2819889 (BioLegend Cat. No. 144717)AB_2819890 (BioLegend Cat. No. 144718)
IsotypeMouse IgG1, κ
ReactivityMouse
ApplicationFC -Quality testedIP, Block -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number3D10
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetLy49H
ImmunogenC1498-Ly49A/H transfectant
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein.  It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice).  Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice.  Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection.  Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).

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