Ultra-LEAF™ Purified anti-mouse TCR γ/δ Antibody, TCR γ/δ, UC7-13D5,BioLegend,107518

Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrostimulation1,2,4. Although several papers have reportedin vivodepletion3, 5,6, another study found that the effect of the antibody could be the functional blocking of the TCR signaling, rather than cell depletion7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos.107515 - 107520).Though clone UC7-13D5 has been used for the depletion of TCR γ/δ T cells in vivo3-5, there is a report suggesting that binding of clone UC7-13D5 to the γ/δ TCR leads to downregulation of the γ/δ TCR, making the γ/δ T cells undetectable although they are still present7.

Host

Armenian Hamster

Reactivity

Mouse

Application

FC -Quality testedIP, Stim, Depletion -Reported in the literature, not verified in house

Platform ID

BAB911087050

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse TCR γ/δ Antibody, TCR γ/δ, UC7-13D5
Cat. No.107518
HostArmenian Hamster
RRIDAB_2813962 (BioLegend Cat. No. 107515)AB_2813963 (BioLegend Cat. No. 107516)AB_2813964 (BioLegend Cat. No. 107517)AB_2813965 (BioLegend Cat. No. 107518)AB_2813966 (BioLegend Cat. No. 107519)AB_2813967 (BioLegend Cat. No. 107520)
IsotypeArmenian Hamster IgG
ReactivityMouse
ApplicationFC -Quality testedIP, Stim, Depletion -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberUC7-13D5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetTCR gamma/delta
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cellsin vitro, and to deplete peripheral TCR-γ/δ-bearing T cellsin vivo.

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