Ultra-LEAF Purified anti-mouse TCR beta chain Antibody, TCR beta chain, H57-597,BioLegend,109254
H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2,in vitrostimulation2,3,in vivodepletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).
Host
Armenian Hamster
Reactivity
Mouse
Application
FC -Quality testedCyTOF® -VerifiedIHC-F, IP, Costim, Depletion -Reported in the literature, not verified in house
Platform ID
BAB727852516
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse TCR beta chain Antibody, TCR beta chain, H57-597
Cat. No.109254
HostArmenian Hamster
RRIDAB_2813970 (BioLegend Cat. No. 109253)AB_2813971 (BioLegend Cat. No. 109254)AB_2813972 (BioLegend Cat. No. 109255)AB_2813973 (BioLegend Cat. No. 109256)AB_2813974 (BioLegend Cat. No. 109257)AB_2813975 (BioLegend Cat. No. 109258)
IsotypeArmenian Hamster IgG
ReactivityMouse
ApplicationFC -Quality testedCyTOF® -VerifiedIHC-F, IP, Costim, Depletion -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberH57-597
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetTCR beta chain
ImmunogenAffinity purified TCR from mouse DO-11.10 cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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