Ultra-LEAF™ Purified anti-mouse TNF-α Antibody, TNF-α, 6B8,BioLegend,510806

ELISA or ELISPOT Capture:The purified 6B8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP6-XT22 antibody (Cat. No. 506312) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Note:For testing mouse TNF-a in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901 to 430906) are specially developed and recommended.

Host

Rat

Reactivity

Mouse

Application

ELISA Capture -Quality testedELISPOT Capture -Reported in the literature, not verified in house

Platform ID

BAB069556006

BioLegend

Headquarters

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Contact

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse TNF-α Antibody, TNF-α, 6B8
Cat. No.510806
HostRat
RRIDAB_2876739 (BioLegend Cat. No. 510805)AB_2876739 (BioLegend Cat. No. 510806)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationELISA Capture -Quality testedELISPOT Capture -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number6B8
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetTNF-alpha
Immunogenrecombinant mouse TNF-α
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell linesin vitro, hemorraghic necrosis of tumorsin vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The 6B8 antibody reacts with mouse and rat tumor necrosis factor-α (TNF-α).

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