Ultra-LEAF Purified anti-mouse alpha-GalCerCD1d complex Antibody, alpha-GalCer:CD1d complex, L363,BioLegend,140517
Additional reported applications (for the relevant formats) include: Surface and intracellular immunofluorescence2and block a-GalCer:mCD1d1 complex induced iNKT activation1.
Host
Mouse
Reactivity
Mouse
Application
FC -Quality testedBlock, ICC -Reported in the literature, not verified in house
Platform ID
BAB931688421
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-mouse alpha-GalCerCD1d complex Antibody, alpha-GalCer:CD1d complex, L363
Cat. No.140517
HostMouse
RRIDAB_2814042 (BioLegend Cat. No. 140517)AB_2814043 (BioLegend Cat. No. 140518)
IsotypeMouse IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedBlock, ICC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberL363
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
Immunogenα-GalCer:mCD1d1 complexes coupled to Mycobacterium tuberculosis PPD
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
The L363 antibody reacts with α-GalCer:mouse CD1d complex. Glycolipid α-galactosylceramide (α-GalCer) is a potent activator of human and murineiNKT cells and is widely used as a model antigen for the studies ofiNKT-mediated immunity. The glycolipid binds to CD1d and forms a complex which is subsequently recognized byiNKT cells, a subset of T cells expressing invariant TCR α chain Vα14, analogous to the peptide-MHC complex recognition by TCR α/ß T cells.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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