Ultra-LEAF Purified anti-mouse/human CD11b Antibody anti-CD11b - M1/70,BioLegend,101270

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4,in vitroblocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Host

Rat

Reactivity

Mouse, Human, Cynomolgus, Rhesus

Application

FC - Quality testedCyTOF® - VerifiedIP, Block, Depletion, IHC-F, ICC - Reported in the literature, not verified in house

Platform ID

BAB560099283

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse/human CD11b Antibody anti-CD11b - M1/70
Cat. No.101270
HostRat
RRIDAB_2813917 (BioLegend Cat. No. 101247)AB_2561479 (BioLegend Cat. No. 101248)AB_2813918 (BioLegend Cat. No. 101269)AB_2813919 (BioLegend Cat. No. 101270)AB_2813920 (BioLegend Cat. No. 101271)AB_2813921 (BioLegend Cat. No. 101272)
IsotypeRat IgG2b, κ
ReactivityMouse, Human, Cynomolgus, Rhesus
ApplicationFC - Quality testedCyTOF® - VerifiedIP, Block, Depletion, IHC-F, ICC - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberM1/70
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD11b
ImmunogenC57BL/10 splenocytes
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

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