Ultra-LEAF™ Purified anti-mouse/human CD44 Antibody, CD44, IM7,BioLegend,103069
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3,in vivoinhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
Host
Rat
Reactivity
Mouse, Human
Application
FC - Quality testedCyTOF® - VerifiedELISA, IHC, IP, CMCD, Stim - Reported in the literature, not verified in house
Platform ID
BAB926390965
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse/human CD44 Antibody, CD44, IM7
Cat. No.103069
HostRat
RRIDAB_2832294 (BioLegend Cat. No. 103065)AB_2561491 (BioLegend Cat. No. 103046)AB_2832295 (BioLegend Cat. No. 103066)AB_2832296 (BioLegend Cat. No. 103067)AB_2832297 (BioLegend Cat. No. 103068)AB_2832298 (BioLegend Cat. No. 103069)
IsotypeRat IgG2b, κ
ReactivityMouse, Human
ApplicationFC - Quality testedCyTOF® - VerifiedELISA, IHC, IP, CMCD, Stim - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberIM7
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD44
ImmunogenDexamethasone-induced myeloid leukemia M1 cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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