Ultra-LEAF™ Purified anti-mouse/rat CD178 (FasL) Antibody, CD178, MFL4,BioLegend,106707

Additional reported applications (for the relevant formats) include:in vitroandin vivoFasL blockade1-4. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Host

Armenian Hamster

Reactivity

Mouse, Rat

Application

FC -Quality testedBlock -Reported in the literature, not verified in house

Platform ID

BAB752940468

BioLegend

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Contact

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse/rat CD178 (FasL) Antibody, CD178, MFL4
Cat. No.106707
HostArmenian Hamster
RRIDAB_2810345 (BioLegend Cat. No. 106707)AB_2810346 (BioLegend Cat. No. 106708)
IsotypeArmenian Hamster IgG
ReactivityMouse, Rat
ApplicationFC -Quality testedBlock -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMFL4
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD178
ImmunogenB6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD178 is a 40 kD member of the TNF/NGF superfamily also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The MFL4 antibody recognizes CD178 in a wide array of mouse strains and has been reported to block CD178/CD95 induced apoptosis. The MFL4 antibody is cross-reactive with rat and mouse FasL.

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