Ultra-LEAF™ Purified anti-mouse/rat TNF-α Antibody, TNF-α, TN3-19.12,BioLegend,506114

ELISA or ELISPOT Capture1:The Ultra-LEAF™ Purified TN3-19.12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated Poly5160 antibody (Cat. No. 516003) as the detecting antibody for detecting mouse/rat TNF-a. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Clone TN3-19.12 has been shown to neutralize murine TNF-a and cross reacts wtih mouse TFN-ß1. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Aside free, 0.2 µm filtered) is recommended for neutralization (Cat. Nos. 506109 - 506114).

Host

Armenian Hamster

Reactivity

Mouse, Rat

Application

ELISA Capture -Quality testedELISPOT Capture, Neut, ICFC, IP, WB -Reported in the literature, not verified in house

Platform ID

BAB157613718

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse/rat TNF-α Antibody, TNF-α, TN3-19.12
Cat. No.506114
HostArmenian Hamster
RRIDAB_2820092 (BioLegend Cat. No. 506109)AB_2820093 (BioLegend Cat. No. 506110)AB_2832810 (BioLegend Cat. No. 506111)AB_2832811 (BioLegend Cat. No. 506112)AB_2832812 (BioLegend Cat. No. 506113)AB_2832813 (BioLegend Cat. No. 506114)
IsotypeArmenian Hamster IgG
ReactivityMouse, Rat
ApplicationELISA Capture -Quality testedELISPOT Capture, Neut, ICFC, IP, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberTN3-19.12
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetTNF-alpha
ImmunogenE. coli-expressed, recombinant mouse TNF-α
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell linesin vitro, hemorraghic necrosis of tumorsin vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-α (TNF-α) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α.

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