Ultra-LEAF Purified anti-mousehuman IL-5 Antibody, IL-5, TRFK5,BioLegend,504315

ELISA1,3,4or ELISPOT2,6Capture:The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.Neutralization1,9:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.Note:For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

Host

Rat

Reactivity

Mouse, Human

Application

ELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, Neut, ICFC, IHC, WB -Reported in the literature, not verified in house

Platform ID

BAB033150960

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mousehuman IL-5 Antibody, IL-5, TRFK5
Cat. No.504315
HostRat
RRIDAB_2814411 (BioLegend Cat. No. 504313)AB_2814412 (BioLegend Cat. No. 504314)AB_2814413 (BioLegend Cat. No. 504315)AB_2814414 (BioLegend Cat. No. 504316)AB_2814415 (BioLegend Cat. No. 504317)AB_2814416 (BioLegend Cat. No. 504318)
IsotypeRat IgG1, κ
ReactivityMouse, Human
ApplicationELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, Neut, ICFC, IHC, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberTRFK5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-5
ImmunogenPartially-purified T cell clone supernatant
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.

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