Ultra-LEAF Purified anti-mouserathuman MCP-1 Antibody, CCL2, 2H5,BioLegend,505911

ELISA or ELISPOT Capture1:The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.Flow Cytometry2:The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1,4,6:The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.Additional reported applications (for the relevant formats) include:Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.

Host

Hamster

Reactivity

Mouse, Rat, Human

Application

ELISA Capture -Quality testedELISPOT Capture, Neut, WB, IHC -Reported in the literature, not verified in house

Platform ID

BAB428451240

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouserathuman MCP-1 Antibody, CCL2, 2H5
Cat. No.505911
HostHamster
RRIDAB_2820086 (BioLegend Cat. No. 505911)AB_2820087 (BioLegend Cat. No. 505912)AB_2820088 (BioLegend Cat. No. 505913)AB_2820089 (BioLegend Cat. No. 505914)AB_2820090 (BioLegend Cat. No. 505915)AB_2820091 (BioLegend Cat. No. 505916)
IsotypeHamster IgG
ReactivityMouse, Rat, Human
ApplicationELISA Capture -Quality testedELISPOT Capture, Neut, WB, IHC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number2H5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCCL2
ImmunogenCHO-expressed, recombinant mouse MCP-1
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

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