VEGF Receptor 2 (D5B1) Rabbit Monoclonal Antibody (Alexa Fluor^®488 Conjugate)#12687,Cell Signaling Technology (CST),12687

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cellsin vitroand angiogenesisin vivo(6-8).Meyer, M. et al. (1999) EMBO J 18, 363-74.Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.Takahashi, T. et al. (2001) EMBO J 20, 2768-78.Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.Alternate NamesCD309; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; FLK1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR; VEGFR-2; VEGFR2; VGFR2

CD309; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; FLK1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR; VEGFR-2; VEGFR2; VGFR2