XBP-1s (E9V3E) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate)#38139,Cell Signaling Technology (CST),38139

XBP-1s (E9V3E) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate) recognizes endogenous levels of total XBP-1s protein. In western blot analysis, this antibody conjgate detects a 78 kDa protein and a 99 kDa protein of unknown identities.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Flow Cytometry (Fixed/Permeabilized): 1:50

Platform ID

BAB207967008

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameXBP-1s (E9V3E) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate)#38139
Cat. No.38139
Accession NumberO35426
Gene ID (Entrez)35426, 22433
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationFlow Cytometry (Fixed/Permeabilized): 1:50
ImmunogenIgG
FormulationSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Following protein synthesis, secretory, intra-organellar, and transmembrane proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. The accumulation of unfolded proteins within the ER triggers an adaptive mechanism known as the unfolded protein response (UPR) that counteracts compromised protein folding (1). The transmembrane serine/threonine kinase IRE1, originally identified inSaccharomyces cerevisiae, is a proximal sensor for the UPR that transmits the unfolded protein signal across the ER membrane (2-4). The human homolog IRE1α was later identified and is ubiquitously expressed in human tissues (5). Upon activation of the unfolded protein response, IRE1α splices X-box binding protein 1 (XBP-1) mRNA through an unconventional mechanism using its endoribonuclease activity (6). This reaction converts XBP-1 from an unspliced XBP-1u isoform to the spliced XBP-1s isoform, which is a potent transcriptional activator that induces expression of many UPR responsive genes (6).Kaufman, R.J. et al. (2002)Nat Rev Mol Cell Biol3, 411-21.Nikawa, J. and Yamashita, S. (1992)Mol Microbiol6, 1441-6.Cox, J.S. et al. (1993)Cell73, 1197-206.Mori, K. et al. (1993)Cell74, 743-56.Tirasophon, W. et al. (1998)Genes Dev12, 1812-24.Lee, K. et al. (2002)Genes Dev16, 452-66.Alternate NamesD11Ertd39; D11Ertd39e; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB; TREB-; TREB-5; Treb5; X-box binding protein 1; X-box-binding protein 1; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; XBP-; XBP-1; XBP-1S; XBP1; XBP1 iso2

Synonyms

D11Ertd39; D11Ertd39e; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB; TREB-; TREB-5; Treb5; X-box binding protein 1; X-box-binding protein 1; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; XBP-; XBP-1; XBP-1S; XBP1; XBP1 iso2

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