anti-human TNF-alpha monoclonal antibody Anti-Human 500 µg,Reliatech,101-M17

Produced in BALB/c mice using highly pure (>98%) recombinant human TNF-alpha as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.

Host

Mouse

Reactivity

Human

Application

ELISA, WB, IHC (P), N

Platform ID

BAB751120193

Reliatech

Headquarters

Lindener Straße 15 38300 Wolfenbüttel Germany

Reliatech

Contact

Tel: +49 (0) 5331 - 8586987
Fax: +49 (0) 5331- 8586989

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

Nameanti-human TNF-alpha monoclonal antibody Anti-Human 500 µg
Cat. No.101-M17
Accession NumberP01375
HostMouse
IsotypeIgG1, kappa
ReactivityHuman
ApplicationELISA, WB, IHC (P), N
Appearance/Formlyophilized from PBS
FormulationPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
StorageThe lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.
Regulatory StatusFor research use only

Scientific Background

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Synonyms

TNF; DIF; TNFA; TNFSF2; TNF-alpha

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