mCherry (E5D8F) Mouse Chimeric Monoclonal Antibody#99913,Cell Signaling Technology (CST),99913

mCherry (E5D8F) Mouse Chimeric Monoclonal Antibody detects mCherry-tagged proteins (either N-terminal tagged or C-terminal tagged) exogenously expressed in cells. Please note that the mCherry tags add approximately 28kDa to the molecular weight of the fusion protein.

Host

Mouse

Reactivity

All Species Expected

Application

Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400

Platform ID

BAB299034539

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamemCherry (E5D8F) Mouse Chimeric Monoclonal Antibody#99913
Cat. No.99913
Accession NumberX5DSL3
HostMouse
SensitivityTransfected Only
ReactivityAll Species Expected
ApplicationImmunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400
Immunogenchimeric
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.mCherry is a monomeric, fluorescent, photostable red dye that was isolated from and further engineered from the Discosoma sea anemone (1). Its peak fluorescent excitation is at 587 nm and emission 610 nm (1).Shaner, N.C. et al. (2004)Nat Biotechnol22, 1567-72.Alternate NamesmCherry; MCherry fluorescent protein

Synonyms

mCherry; MCherry fluorescent protein

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