Antibody reagents are core probes for flow cytometry to achieve accurate single‑cell identification and quantitative analysis. Based on specific antigen‑antibody binding and fluorescent labeling, they convert target molecules on cell surfaces or intracellularly into detectable fluorescent signals. These reagents enable precise identification of immune cell subsets, determination of cell activation, apoptosis and cycle status, and support multicolor detection and fluorescence‑activated cell sorting (FACS). They provide highly specific and sensitive single‑cell quantitative data for immune phenotyping, tumor monitoring, stem cell characterization, drug efficacy evaluation and clinical diagnosis, serving as key tools for extending flow cytometry from physical characterization to molecular functional analysis.
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