Search results for 603351

Purified anti-RIP-1 Antibody, RIP-1, W17101A, BioLegend, 603351

This clone was tested for ICC using HeLa cells with localization expected in the cytosol and plasma membrane. Three fix/perm methods were used: methanol fixation, or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Methanol-only fixation produced a strong signal with the expected cytoplasmic localization. PFA-fixation followed by permeabilization with either methanol or Triton X-100 did not produce the expected cytoplasmic/membrane signal.This clone was tested for IHC-P on human skin tissue using Biolegend’s AEC Chromagen. Positive signal was not observed.