Search results for 947206

Alexa Fluor® 647 anti-FOXO1 Antibody, FOXO1, W20064D, BioLegend, 947206

This clone was tested for ICC using serum-starved HeLa cells treated with insulin and untreated serum-starved HeLa cells. Staining was expected to localize to the nucleus prior to treatment and localize to the cytoplasm following treatment. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced a strong signal and the expected shift in localization.This clone was tested for IHC on human melanoma tissue using Biolegend’s DAB Chromagen. No staining was produced.