Search results for Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) Antibody, AMPKα Phospho (Thr172), A20017A
Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) Antibody, AMPKα Phospho (Thr172), A20017A, BioLegend, 600655
This clone was tested for ICC using serum-starved untreated HEK293 cells (negative control) and HEK293 cells treated with 5.0 µM oligomycin for 30 minutes (positive control). Staining was expected to localize to the nucleus and cytoplasm. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Both PFA fixation followed by methanol permeabilization and PFA fixation followed by Triton X-100 permeabilization produced a strong signal with the expected localization. Methanol-only fixation only produced a faint signal.
Host
Mouse
Reactivity
Human, Mouse
Applications
IHC-P - Quality testedICFC - Verified
Conjugation
Alexa Fluor® 647 anti-AMPKα Phospho (Thr172) Antibody, AMPKα Phospho (Thr172), A20017A, BioLegend, 600656
This clone was tested for ICC using serum-starved untreated HEK293 cells (negative control) and HEK293 cells treated with 5.0 µM oligomycin for 30 minutes (positive control). Staining was expected to localize to the nucleus and cytoplasm. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Both PFA fixation followed by methanol permeabilization and PFA fixation followed by Triton X-100 permeabilization produced a strong signal with the expected localization. Methanol-only fixation only produced a faint signal.
Host
Mouse
Reactivity
Human, Mouse
Applications
IHC-P - Quality testedICFC - Verified
Conjugation
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