Search results for Alexa Fluor 647 anti-Neurofilament H
Alexa Fluor 647 anti-Neurofilament H, Nonphosphorylated Antibody - SMI 32, BioLegend, 801711
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality tested
Conjugation
Alexa Fluor 647 anti-Neurofilament H, Nonphosphorylated Antibody - SMI 32, BioLegend, 801712
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality tested
Conjugation
Alexa Fluor 647 anti-Neurofilament H & M NF-H/NF-M, Phosphorylated Antibody - SMI 310, BioLegend, 837709
Additional reported applications (for the relevant formats) include: Western blotting4, immunocytochemistry2, immunohistochemical staining of frozen tissue sections1,3, and spatial biology (IBEX)5,6.Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedSB -Reported in the literature, not verified in house
Conjugation
Alexa Fluor 647 anti-Neurofilament H & M NF-H/NF-M, Phosphorylated Antibody - SMI 310, BioLegend, 837710
Additional reported applications (for the relevant formats) include: Western blotting4, immunocytochemistry2, immunohistochemical staining of frozen tissue sections1,3, and spatial biology (IBEX)5,6.Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedSB -Reported in the literature, not verified in house
Conjugation
Alexa Fluor 647 anti-Neurofilament H NF-H, Phosphorylated Antibody, Neurofilament H Phospho NF-H, SMI 31, BioLegend, 801613
Additional reported applications (for the relevant formats) include: Western blotting1, immunohistochemistry2,4, and immunocytochemistry4.SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality tested
Conjugation
Alexa Fluor 647 anti-Neurofilament H NF-H, Phosphorylated Antibody, Neurofilament H Phospho NF-H, SMI 31, BioLegend, 801614
Additional reported applications (for the relevant formats) include: Western blotting1, immunohistochemistry2,4, and immunocytochemistry4.SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality tested
Conjugation
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