Search results for Biotin anti-Neurofilament H
Biotin anti-Neurofilament H (NF-H), Phosphorylated Antibody - SMI 31, BioLegend, 801608
Additional reported applications (for the relevant formats) include: Western blotting1, immunohistochemistry2,4, and immunocytochemistry4.SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P - Quality testedWB - Verified
Conjugation
Biotin anti-Neurofilament H M (NF-H/NF-M), Hypophosphorylated - anti-Neurofilament H M (NF-H/NF-M), Phospho - SMI 35, BioLegend, 835606
Additional reported applications (for the relevant formats of this clone): include ELISA Capture1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)6,7.Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
WB -Quality tested
Conjugation
Biotin anti-Neurofilament H & M NF-H/NF-M, Phosphorylated Antibody anti-Neurofilament H & M (NF-H/NF-M), Phospho - SMI 310, BioLegend, 837706
Additional reported applications (for the relevant formats) include: Western blotting4, immunocytochemistry2and immunohistochemical staining of frozen tissue sections1, 3.Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
WB - Quality tested
Conjugation
Please provide the required information below so that we can quickly source your products.