Search results for Purified anti-AMPKα Phospho (Thr172) Antibody, Aquaporin-4, A20017A
Purified anti-AMPKα Phospho (Thr172) Antibody, Aquaporin-4, A20017A, BioLegend, 600651
This clone was tested for ICC using serum-starved untreated HEK293 cells (negative control) and HEK293 cells treated with 5.0 µM oligomycin for 30 minutes (positive control). Staining was expected to localize to the nucleus and cytoplasm. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Both PFA fixation followed by methanol permeabilization and PFA fixation followed by Triton X-100 permeabilization produced a strong signal with the expected localization. Methanol-only fixation only produced a faint signal.
Host
Mouse
Reactivity
Human, Mouse
Applications
WB - Quality testedICC, ICFC, IHC-P - Verified
Conjugation
Purified anti-AMPKα Phospho (Thr172) Antibody, Aquaporin-4, A20017A, BioLegend, 600652
This clone was tested for ICC using serum-starved untreated HEK293 cells (negative control) and HEK293 cells treated with 5.0 µM oligomycin for 30 minutes (positive control). Staining was expected to localize to the nucleus and cytoplasm. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Both PFA fixation followed by methanol permeabilization and PFA fixation followed by Triton X-100 permeabilization produced a strong signal with the expected localization. Methanol-only fixation only produced a faint signal.
Host
Mouse
Reactivity
Human, Mouse
Applications
WB - Quality testedICC, ICFC, IHC-P - Verified
Conjugation
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