Search results for Purified anti-CHD4 Antibody, CHD4, W18333A
Purified anti-CHD4 Antibody, CHD4, W18333A, BioLegend, 942301
During product development testing for western blot, we observed additional bands below the predominant, full-length CHD4 band. These additional bands were also detected using a CHD4 antibody raised using a separate immunogen. We therefore believe these bands represent CHD4 degradation products or additional CHD4 isoforms.This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. While both methods were compatible with robust CHD4 staining, we observed punctate, extranuclear staining of cells permeabilzed with Triton X-100. We therefore do not recommend this method when using the clone for ICC.
Host
Rat
Reactivity
Human, Mouse
Applications
WB - Quality testedICC - Verified
Conjugation
Purified anti-CHD4 Antibody, CHD4, W18333A, BioLegend, 942302
During product development testing for western blot, we observed additional bands below the predominant, full-length CHD4 band. These additional bands were also detected using a CHD4 antibody raised using a separate immunogen. We therefore believe these bands represent CHD4 degradation products or additional CHD4 isoforms.This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. While both methods were compatible with robust CHD4 staining, we observed punctate, extranuclear staining of cells permeabilzed with Triton X-100. We therefore do not recommend this method when using the clone for ICC.
Host
Rat
Reactivity
Human, Mouse
Applications
WB - Quality testedICC - Verified
Conjugation
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