Search results for Purified anti-ERG Antibody, ERG, W19256A
Purified anti-ERG Antibody, ERG, W19256A, BioLegend, 940201
This clone was tested for ICC using HeLa (low expressorl) and MOLT-4 (high expressor) cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in robust ERG staining.This clone does not recognize mouse ER.When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor ERG staining.True-Nuclear™ Transcription Factor Buffer Set gave a better signal to noise ratio at all tested concentrations when compared to the True Phos perm buffer.
Host
Rat
Reactivity
Human
Applications
WB - Quality testedICC, IP, ICFC - Verified
Conjugation
Purified anti-ERG Antibody, ERG, W19256A, BioLegend, 940202
This clone was tested for ICC using HeLa (low expressorl) and MOLT-4 (high expressor) cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in robust ERG staining.This clone does not recognize mouse ER.When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor ERG staining.True-Nuclear™ Transcription Factor Buffer Set gave a better signal to noise ratio at all tested concentrations when compared to the True Phos perm buffer.
Host
Rat
Reactivity
Human
Applications
WB - Quality testedICC, IP, ICFC - Verified
Conjugation
Please provide the required information below so that we can quickly source your products.