Search results for Purified anti-FBXL5 Antibody, FBXL5, 3F5G12G9
Purified anti-FBXL5 Antibody, FBXL5, 3F5G12G9, BioLegend, 672703
This clone was tested for ICC on PFA-fixed, Triton X-100-permeabilized NIH/3T3 cells. The clone produced nuclear staining that did not match the iron-responsiveness observed during western blotting. Because of this, we do not recommend using this clone for IF.During product development testing, a protein with a slightly lower molecular weight than the target was observed during PD testing. This protein displayed a similar iron-responsiveness to the target band. It is unclear whether this band represents a minor FBXL5 degradation product or a truncated isoform.This clone does not recognize human FBXL5. For detection of human FBXL5 by western blot, we recommend BioLegend clone 10F4H9D12 (Cat. No. 672604).This clone has not been tested for IP.
Host
Mouse
Reactivity
Mouse
Applications
WB -Quality tested
Conjugation
Purified anti-FBXL5 Antibody, FBXL5, 3F5G12G9, BioLegend, 672704
This clone was tested for ICC on PFA-fixed, Triton X-100-permeabilized NIH/3T3 cells. The clone produced nuclear staining that did not match the iron-responsiveness observed during western blotting. Because of this, we do not recommend using this clone for IF.During product development testing, a protein with a slightly lower molecular weight than the target was observed during PD testing. This protein displayed a similar iron-responsiveness to the target band. It is unclear whether this band represents a minor FBXL5 degradation product or a truncated isoform.This clone does not recognize human FBXL5. For detection of human FBXL5 by western blot, we recommend BioLegend clone 10F4H9D12 (Cat. No. 672604).This clone has not been tested for IP.
Host
Mouse
Reactivity
Mouse
Applications
WB -Quality tested
Conjugation
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