Search results for Purified anti-Lck Antibody anti-Lck - A17013D
Purified anti-Lck Antibody anti-Lck - A17013D, BioLegend, 601001
Clone A17013D was derived from a human Lck peptide phosphorylated at Y505 immunogen, but still displayed pan reactivity towards the unmodified peptide during western blot screening. This clone was moderately stronger than BioLegend clone LCK-01 for western blot and substantially stronger for immunofluorescence microscopy in parallel testing. But it shows a weak affinity towards mouse Lck. This clone is predicted to recognize both 58 and 61 kD isoforms based off the presence of the immunizing epitope in both proteins and may also recognize certain SRC tyrosine kinase family members due to high sequence similarity.Phosphorylation of Lck Y505 reduces binding of this antibody by western blot (please see Fig. 3); we recommend LCK-01 as a pan control antibody.The additional band of ~ 50 kD detected bellow the canonical Lck band in IP experiment could be Lck degradation product.
Host
Mouse
Reactivity
Human, Mouse
Applications
WB -Quality testedICC, IP -Verified
Conjugation
Purified anti-Lck Antibody anti-Lck - A17013D, BioLegend, 601002
Clone A17013D was derived from a human Lck peptide phosphorylated at Y505 immunogen, but still displayed pan reactivity towards the unmodified peptide during western blot screening. This clone was moderately stronger than BioLegend clone LCK-01 for western blot and substantially stronger for immunofluorescence microscopy in parallel testing. But it shows a weak affinity towards mouse Lck. This clone is predicted to recognize both 58 and 61 kD isoforms based off the presence of the immunizing epitope in both proteins and may also recognize certain SRC tyrosine kinase family members due to high sequence similarity.Phosphorylation of Lck Y505 reduces binding of this antibody by western blot (please see Fig. 3); we recommend LCK-01 as a pan control antibody.The additional band of ~ 50 kD detected bellow the canonical Lck band in IP experiment could be Lck degradation product.
Host
Mouse
Reactivity
Human, Mouse
Applications
WB -Quality testedICC, IP -Verified
Conjugation
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